A phylogenetic framework of the legume genus Aeschynomene for comparative genetic analysis of the Nod-dependent and Nod-independent symbioses

Laurent Brottier; Clémence Chaintreuil; Paul Simion; Celine Scornavacca; Ronan Rivallan; Pierre Mournet; Lionel Moulin; Gwilym Lewis; Joël Fardoux; Spencer Brown; Mario Gomez-Pacheco; Mickaël Bourges; Catherine Hervouet; Mathieu Gueye; Robin Duponnois; Heriniaina Ramanankierana; Herizo Randriambanona; Hervé Vandrot; Maria Zabaleta; Maitrayee Dasgupta; Angélique D’hont; Eric Giraud; Jean-François Arrighi

doi:10.1186/s12870-018-1567-z

Article dans une revue

A phylogenetic framework of the legume genus Aeschynomene for comparative genetic analysis of the Nod-dependent and Nod-independent symbioses

Laurent Brottier ¹ Clémence Chaintreuil ^{1, 2} Paul Simion ³ Celine Scornavacca ³ Ronan Rivallan ⁴ Pierre Mournet ⁴ Lionel Moulin ¹ Gwilym Lewis ⁵ Joël Fardoux ^{1, 2} Spencer Brown ⁶ Mario Gomez-Pacheco ⁷ Mickaël Bourges ⁷ Catherine Hervouet ⁸ Mathieu Gueye ⁹ Robin Duponnois ^{1, 2} Heriniaina Ramanankierana ¹⁰ Herizo Randriambanona ¹¹ Hervé Vandrot ^{12, 13} Maria Zabaleta ¹⁴ Maitrayee Dasgupta ¹⁴ Angélique D’hont ⁴ Eric Giraud ^{1, 2} Jean-François Arrighi ^{1, 2}

1 UMR LSTM - Laboratoire des symbioses tropicales et méditerranéennes

2 IRD - Institut de Recherche pour le Développement

3 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier

4 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales

5 Royal Botanic Gardens Kew

6 ISV - Institut des sciences du végétal

7 I2BC - Institut de Biologie Intégrative de la Cellule

8 TIRO - UMR E4320 - Transporteurs en Imagerie et Radiothérapie en Oncologie

SHFJ - Service Hospitalier Frédéric Joliot, TIRO-MATOs - UMR E4320 : UMR E4320

9 IFAN - Institut Fondamental d'Afrique Noire - Laboratoire de botanique

10 Centre National de Recherches sur l'Environnement

11 Centre National de Recherche sur l'Environnement

12 IAC - Institut Agronomique Néo-Calédonien

13 UMR AMAP - Botanique et Modélisation de l'Architecture des Plantes et des Végétations

14 IIBCE - Instituto de Investigaciones Biológicas Clemente Estable [Montevideo]

Abstract : Background: Among semi-aquatic species of the legume genus Aeschynomene, some have the property of being nodulated by photosynthetic Bradyrhizobium lacking the nodABC genes necessary for the synthesis of Nod factors. Knowledge of the specificities underlying this Nod-independent symbiosis has been gained from the model legume Aeschynomene evenia but our understanding remains limited due to the lack of comparative genetics with related taxa using a Nod factor-dependent process. To fill this gap, we combined different approaches to perform a thorough comparative analysis in the genus Aeschynomene. Results: This study significantly broadened previous taxon sampling, including in allied genera, in order to construct a comprehensive phylogeny. In the phylogenetic tree, five main lineages were delineated, including a novel lineage, the Nod-independent clade and another one containing a polytomy that comprised several Aeschynomene groups and all the allied genera. This phylogeny was matched with data on chromosome number, genome size and low-copy nuclear gene sequences to reveal the diploid species and a polytomy containing mostly polyploid taxa. For these taxa, a single allopolyploid origin was inferred and the putative parental lineages were identified. Finally, nodulation tests with different Bradyrhizobium strains revealed new nodulation behaviours and the diploid species outside of the Nod-independent clade were compared for their experimental tractability and genetic diversity. Conclusions: The extended knowledge of the genetics and biology of the different lineages sheds new light of the evolutionary history of the genus Aeschynomene and they provide a solid framework to exploit efficiently the diversity encountered in Aeschynomene legumes. Notably, our backbone tree contains all the species that are diploid and it clarifies the genetic relationships between the Nod-independent clade and the Nod-dependent lineages. This study enabled the identification of A. americana and A. patula as the most suitable species to undertake a comparative genetic study of the Nod-independent and Nod-dependent symbioses.

Keywords : Symbiosis Fabaceae Phylogenetics Polyploidy Nodulation Aeschynomene Genetics Legumes

Domaine :

Sciences du Vivant [q-bio] / Biodiversité / Systématique, phylogénie et taxonomie

Sciences du Vivant [q-bio] / Ecologie, Environnement / Ecosystèmes

Sciences du Vivant [q-bio] / Biologie végétale / Botanique

Sciences de l'environnement / Biodiversité et Ecologie

Liste complète des métadonnées

Littérature citée [58 références] Voir Masquer Télécharger

https://hal.umontpellier.fr/hal-01961745
Contributeur : Yannick Brohard <>
Soumis le : jeudi 20 décembre 2018 - 10:45:24
Dernière modification le : mercredi 3 juin 2020 - 23:26:01
Document(s) archivé(s) le : vendredi 22 mars 2019 - 11:58:34

Brottier_etal_BMC_Pl_Biol_2018...

Fichiers éditeurs autorisés sur une archive ouverte

HAL Id : hal-01961745, version 1
IRD : fdi:010074749
DOI : 10.1186/s12870-018-1567-z
PRODINRA : 456222
WOS : 000452283100004
PUBMED : 30518342

A phylogenetic framework of the legume genus Aeschynomene for comparative genetic analysis of the Nod-dependent and Nod-independent symbioses

Fichier

Licence

Identifiants

Collections

Citation

Exporter

Métriques

389

377

A phylogenetic framework of the legume genus Aeschynomene for comparative genetic analysis of the Nod-dependent and Nod-independent symbioses

Fichier

Licence

Identifiants

Collections

Citation

Exporter

Partager

Métriques

389

377