Communication Dans Un Congrès Année : 2022

Kinetics of stator recruitment and release in the bacterial flagellar motor

Ashley Nord

Résumé

The bacterial flagellar motor is the membrane-embedded rotary motor, which turns the flagellum that provides thrust to many bacteria. This large multimeric complex, composed of a few dozen constituent proteins, is a hallmark of dynamic subunit exchange. The stator units are inner-membrane ion channels that dynamically bind to the peptidoglycan at the rotor periphery and apply torque. Their dynamic exchange is a function of the viscous load on the flagellum, allowing the bacterium to adapt to its local environment, although the molecular mechanisms of mechanosensitivity remain unknown. Here, by actively perturbing the steady-state stator stoichiometry of individual motors, we reveal a stoichiometry-dependent asymmetry in stator remodeling kinetics. We interrogate the potential effect of next-neighbor interactions and local stator unit depletion and find that neither can explain the observed asymmetry. We then simulate and fit two mechanistically diverse models that recapitulate the asymmetry, finding assembly dynamics to be particularly well described by a two-state catch-bond mechanism. https://doi.org/10.1126/sciadv.abl8112
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Dates et versions

hal-04901816 , version 1 (20-01-2025)

Identifiants

  • HAL Id : hal-04901816 , version 1

Citer

Maria-José Franco-Oñate, Ruben Perez-Carrasco, Jean-Charles Walter, Jerome Dorignac, Frederic Geniet, et al.. Kinetics of stator recruitment and release in the bacterial flagellar motor. International Physics of Living Systems (IPOLS) 2022, CNRS, May 2022, Montpellier, France. ⟨hal-04901816⟩
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