In the face of the undesirable effects induced by anti-inflammatory drugs, there has been a return, nowadays, to the search for active ingredients based on plants. Herein, for the first time we study the anti-inflammatory activity of essential oils of three species of the genus Inula: Inula viscosa, Inula graveolens and Inula crithmoides in lipopolysaccharide (LPS)-activated macrophages. Essential oils have shown excellent preventive anti-inflammatory potential by causing inhibition of nitric oxide (NO) production in LPSactivated RAW264.7 macrophages with IC50s ranging between 15 and 35 µg  mL− 1. On the other hand, the major acidic compounds, more precisely α- and β-costic acids, have been isolated from Inula viscosa and Inula graveolens essential oils and evaluated for their anti-inflammatory effect. These compounds appear to have a moderate preventive inhibitory effect on NO production relative to the significant effect generated by the neutral minority components present in the oils such as borneol, bornyl acetate, (E)-nerolidol, caryophyllene oxide, T-cadinol and eugenol. Therefore, we can deduce that the studied essential oils could be used as anti-inflammatory agents for the treatment of various inflammatory pathologies.