Multiple parameters shape the 3D chromatin structure of single nuclei - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2022

Multiple parameters shape the 3D chromatin structure of single nuclei

Résumé

Abstract The spatial organization of chromatin at the scale of topologically associating domains (TADs) and below displays large cell-to-cell variations. Up until now, how this heterogeneity in chromatin conformation is shaped by chromatin condensation, TAD insulation, and transcription has remained mostly elusive. Here, we used Hi-M, a multiplexed DNA-FISH imaging technique providing developmental timing and transcriptional status, to show that the emergence of TADs at the ensemble level partially segregates the conformational space explored by single nuclei during the early development of Drosophila embryos. Surprisingly, a substantial fraction of nuclei displayed strong insulation even before TADs emerged. Moreover, active transcription within a TAD led to minor changes to the local inter- and intra-TAD chromatin conformation in single nuclei and only weakly affected insulation to the neighboring TAD. Overall, our results indicate that multiple parameters contribute to shaping the chromatin architecture of single nuclei at the TAD scale.
Fichier principal
Vignette du fichier
Goetz_et_al-2022-Nature_Communications.pdf (3.87 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04216523 , version 1 (25-09-2023)

Identifiants

Citer

Markus Götz, Olivier Messina, Sergio Espinola, Jean-Bernard Fiche, Marcelo Nollmann. Multiple parameters shape the 3D chromatin structure of single nuclei. Nature Communications, 2022, 13, ⟨10.1101/2022.01.16.476319⟩. ⟨hal-04216523⟩
5 Consultations
3 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More