The Ets2 Transcription Factor Inhibits Apoptosis Induced by Colony-Stimulating Factor 1 Deprivation of Macrophages through a Bcl-x L -Dependent Mechanism - Université de Montpellier
Article Dans Une Revue Molecular and Cellular Biology Année : 1999

The Ets2 Transcription Factor Inhibits Apoptosis Induced by Colony-Stimulating Factor 1 Deprivation of Macrophages through a Bcl-x L -Dependent Mechanism

Résumé

ABSTRACT Bcl-x L , a member of the Bcl-2 family, inhibits apoptosis, and its expression is regulated at the transcriptional level, yet nothing is known about the transcription factors specifically activating this promoter. The bcl-x promoter contains potential Ets binding sites, and we show that the transcription factor, Ets2, first identified by its sequence identity to v- ets of the E26 retrovirus, can transactivate the bcl-x promoter. Transient expression of Ets2 results in the upregulation of Bcl-x L but not of Bcl-x S , an alternatively spliced gene product which induces apoptosis. Ets2 is ubiquitously expressed at low levels in a variety of cell types and tissues but is specifically induced to abundant levels during macrophage differentiation. Since Bcl-x L is also upregulated during macrophage differentiation, we asked whether the bcl-x could be a direct downstream target gene of Ets2 in macrophages. BAC1.2F5 macrophages, which are dependent on macrophage colony-stimulating factor 1 (CSF-1) for their growth and survival, were used in these studies. We show that CSF-1 stimulation of BAC1.2F5 macrophages results in the upregulation of expression of ets2 and bcl-x L with similar kinetics of induction. In the absence of CSF-1, these macrophages undergo cell death by apoptosis, whereas constitutive expression of Ets2 rescues these cells from cell death, and bcl-x L is upregulated. These results strongly suggest a novel role of Ets2 in affecting apoptosis through its regulation of Bcl-x L transcription.

Dates et versions

hal-03763152 , version 1 (29-08-2022)

Identifiants

Citer

Lidia Sevilla, Christel Aperlo, Vjekoslav Dulic, Jean Claude Chambard, Christel Boutonnet, et al.. The Ets2 Transcription Factor Inhibits Apoptosis Induced by Colony-Stimulating Factor 1 Deprivation of Macrophages through a Bcl-x L -Dependent Mechanism. Molecular and Cellular Biology, 1999, 19 (4), pp.2624-2634. ⟨10.1128/MCB.19.4.2624⟩. ⟨hal-03763152⟩
16 Consultations
0 Téléchargements

Altmetric

Partager

More