Sexual prejudice and its negative consequences remain major issues in Western societies, and numerous studies have tried to pinpoint its sociocultural underpinnings. However, most research has operationalized sexual prejudice via self-report measures or via implicit association tests (IATs), although it surfaces in language use and can be traced in spontaneous speech. Here, we report results from an experimental study investigating sexual prejudice in a corpus of spontaneous speech samples. Specifically, we tested in a context-sensitive sentiment analysis approach which attitudes (negative vs. positive) and emotions ( joy, sadness, anger, fear, disgust ) were voiced by the participants in response to picture prompts displaying homosexual couples. We also considered the sociocultural basis of prejudicial attitudes, in particular the effects of the participants’ cultural background (France vs. Maghreb), age and gender. We find strong effects of cultural background and gender both on the frequency of negative vs. positive attitudes expressed, and on discrete emotion categories, namely that male Maghrebian participants were more negative and conveyed more fear . The results are discussed in the context of current diversity approaches in France and their implications for potential prejudice regulation strategies. We further discuss in how far our context-sensitive sentiment analysis approach advances research on sexual prejudice.