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Article Dans Une Revue Angewandte Chemie International Edition Année : 2015

A Fluorinated Detergent for Membrane-Protein Applications


Surfactants carrying fluorocarbon chains hold great promise as gentle alternatives to conventional hydrocarbon-based detergents for the solubilization and handling of integral membrane proteins. However, their inertness towards lipid bilayer membranes has limited the usefulness of fluorinated surfactants in situations where detergent-like activity is required. We demonstrate that fluorination does not necessarily preclude detergency, as exemplified by a fluorinated octyl maltoside derivative termed F6 OM. This nonionic compound readily interacts with and completely solubilizes phospholipid vesicles in a manner reminiscent of conventional detergents without, however, compromising membrane order at subsolubilizing concentrations. Owing to this mild and unusual mode of detergency, F6 OM outperforms a lipophobic fluorinated surfactant in chaperoning the functional refolding of an integral membrane enzyme by promoting bilayer insertion in the absence of micelles.



Dates et versions

hal-03560145 , version 1 (07-02-2022)



Erik Frotscher, Bartholomäus Danielczak, Carolyn Vargas, Annette Meister, Grégory Durand, et al.. A Fluorinated Detergent for Membrane-Protein Applications. Angewandte Chemie International Edition, 2015, 54 (17), pp.5069-5073. ⟨10.1002/anie.201412359⟩. ⟨hal-03560145⟩
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