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Article Dans Une Revue Current Protocols in Nucleic Acid Chemistry Année : 2017

Water‐Medium Synthesis of Nucleoside 5′‐Polyphosphates


This unit describes a one-pot, two step synthesis of ribonucleoside 5'-di- and 5'-triphosphates, as well as their purification. The first step of the synthesis involves the activation of an unprotected ribonucleoside 5'-monophosphate with 2-chloro-1,3-dimethylimidazolinium hexafluorophosphate and imidazole, in a mixture of water/acetonitrile. The resulting phosphorimidazolate intermediate is then treated with inorganic phosphate or pyrophosphate to afford the corresponding nucleoside 5'-di- or 5'-triphosphates. The attractive features of this strategy include the absence of protecting groups on the starting material and convenient set up (i.e., use of water, non-dry solvents and reagents, commercially available sodium salts). © 2017 by John Wiley & Sons, Inc.


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hal-03557532 , version 1 (04-02-2022)



Anaïs Depaix, Suzanne Peyrottes, Béatrice Roy. Water‐Medium Synthesis of Nucleoside 5′‐Polyphosphates. Current Protocols in Nucleic Acid Chemistry, 2017, 69 (1), pp.13.16.1-13.16.11. ⟨10.1002/cpnc.30⟩. ⟨hal-03557532⟩
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