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Article Dans Une Revue Open Biology Année : 2015

The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth

Résumé

PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell-wall biosynthesis. Here we demonstrate that overexpression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to β-lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in cell-wall biosynthesis as revealed by proteomics studies. The growth inhibition caused by overexpression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions, but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis , and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell-wall architecture.
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hal-03472298 , version 1 (01-06-2022)

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Obolbek Turapov, Jessica Loraine, Christopher Jenkins, Philippe Barthe, Daniel Mcfeely, et al.. The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth. Open Biology, 2015, 5 (7), pp.150025. ⟨10.1098/rsob.150025⟩. ⟨hal-03472298⟩
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