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Article Dans Une Revue Annals of Neurology Année : 2019

Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies

Lynne Krohn (1, 2) , Tuğba Nur Öztürk (3, 4, 5) , Benoît Vanderperre (2, 6) , Bouchra Ouled Amar Bencheikh (2, 6) , Jennifer Ruskey (2, 6) , Sandra Laurent (2, 6) , Dan Spiegelman (2, 6) , Ronald Postuma (2, 6) , Isabelle Arnulf (7, 8) , Michele Hu (9, 10) , Yves Dauvilliers (11, 12) , Birgit Högl (13) , Ambra Stefani (13) , Christelle Charley Monaca (14) , Giuseppe Plazzi (15, 16) , Elena Antelmi (15, 16) , Luigi Ferini-Strambi (17) , Anna Heidbreder (18) , Uladzislau Rudakou (1, 2) , Valérie Cochen de Cock (19, 20) , Peter Young (21) , Pavlina Wolf (22) , Petra Oliva (22) , Xiaokui Kate Zhang (22) , Lior Greenbaum (23, 24, 25) , Christopher Liong (26) , Jean-François Gagnon (27, 28) , Alex Desautels (27, 29) , Sharon Hassin-Baer (25, 26, 24) , Jacques Montplaisir (27, 30) , Nicolas Dupré (29, 31) , Guy Rouleau (1, 2, 32) , Edward Fon (2, 32) , Jean-François Trempe (33) , Guillaume Lamoureux (3, 4, 5, 34) , Roy Alcalay (26, 27) , Ziv Gan-Or (1, 2, 6)
1 Department of Human Genetics [Montréal]
2 Montreal Neurological Institute and Hospital
3 Concordia University [Montreal]
4 Centre for Research in Molecular Modeling and Department of Chemistry & Biochemistry
5 PROTEO, The Quebec Network for Research on Protein Function, Engineering, and Applications
6 Department of Neurology and Neurosurgery [Montreal]
7 CHU Pitié-Salpêtrière [AP-HP]
8 ICM - Institut du Cerveau = Paris Brain Institute
9 Department of Physiology, Anatomy and Genetics [Oxford]
10 Nuffield Department of Clinical Neurosciences [Oxford]
11 Hôpital Gui de Chauliac [CHU Montpellier]
12 PSNREC - Neuropsychiatrie : recherche épidémiologique et clinique
13 Universität Innsbruck [Innsbruck]
14 CHRU Lille - Service de neurophysiologie clinique
15 UNIBO - Alma Mater Studiorum Università di Bologna = University of Bologna
16 Institute of Neurological Sciences of Bologna IRCCS
17 UniSR - Universita Vita Salute San Raffaele = Vita-Salute San Raffaele University [Milan, Italie]
18 WWU - Westfälische Wilhelms-Universität Münster = University of Münster
19 Clinique Beau Soleil [Montpellier]
20 EuroMov - Euromov
21 UKM - University Hospital Münster - Universitaetsklinikum Muenster [Germany]
22 Sanofi Genzyme
23 The Danek Gertner Institute of Genetics, Chaim Sheba Medical Center
24 Chaim Sheba Medical Center
25 Sackler Faculty of Medicine
26 Columbia University College of Physicians and Surgeons
27 Center for advanced research in sleep medicine, Montreal
28 UQAM - Université du Québec à Montréal = University of Québec in Montréal
29 Axe Neurosciences [CHU Québec]
30 Research Center of CIUSSS of the North of Montreal and Department of Psychiatry, University of Montreal, Montreal, Quebec, Canada
31 Laval University Medical center
32 MUHC - McGill University Health Center [Montreal]
33 Department of Pharmacology and Therapeutics [Montréal]
34 CCIB - Center for Computational and Integrative Biology [Camden]
Lynne Krohn
Department of Human Genetics [Montréal]
Montreal Neurological Institute and Hospital
Tuğba Nur Öztürk
  • Fonction : Auteur
Concordia University [Montreal]
Centre for Research in Molecular Modeling and Department of Chemistry & Biochemistry
PROTEO, The Quebec Network for Research on Protein Function, Engineering, and Applications
Benoît Vanderperre
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Bouchra Ouled Amar Bencheikh
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Jennifer Ruskey
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Sandra Laurent
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Dan Spiegelman
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Ronald Postuma
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]
Isabelle Arnulf
CHU Pitié-Salpêtrière [AP-HP]
Institut du Cerveau = Paris Brain Institute
Michele Hu
  • Fonction : Auteur
Department of Physiology, Anatomy and Genetics [Oxford]
Nuffield Department of Clinical Neurosciences [Oxford]
Yves Dauvilliers
Hôpital Gui de Chauliac [CHU Montpellier]
Neuropsychiatrie : recherche épidémiologique et clinique
Birgit Högl
  • Fonction : Auteur
Universität Innsbruck [Innsbruck]
Ambra Stefani
Universität Innsbruck [Innsbruck]
Christelle Charley Monaca
  • Fonction : Auteur
Service de neurophysiologie clinique
Giuseppe Plazzi
Alma Mater Studiorum Università di Bologna = University of Bologna
Institute of Neurological Sciences of Bologna IRCCS
Elena Antelmi
Alma Mater Studiorum Università di Bologna = University of Bologna
Institute of Neurological Sciences of Bologna IRCCS
Luigi Ferini-Strambi
Universita Vita Salute San Raffaele = Vita-Salute San Raffaele University [Milan, Italie]
Anna Heidbreder
Westfälische Wilhelms-Universität Münster = University of Münster
Uladzislau Rudakou
Department of Human Genetics [Montréal]
Montreal Neurological Institute and Hospital
Valérie Cochen de Cock
Clinique Beau Soleil [Montpellier]
Euromov
Peter Young
  • Fonction : Auteur
University Hospital Münster - Universitaetsklinikum Muenster [Germany]
Pavlina Wolf
  • Fonction : Auteur
Sanofi Genzyme
Petra Oliva
  • Fonction : Auteur
Sanofi Genzyme
Xiaokui Kate Zhang
  • Fonction : Auteur
Sanofi Genzyme
Lior Greenbaum
  • Fonction : Auteur
The Danek Gertner Institute of Genetics, Chaim Sheba Medical Center
Chaim Sheba Medical Center
Sackler Faculty of Medicine
Christopher Liong
  • Fonction : Auteur
Columbia University College of Physicians and Surgeons
Jean-François Gagnon
  • Fonction : Auteur
Center for advanced research in sleep medicine, Montreal
Université du Québec à Montréal = University of Québec in Montréal
Alex Desautels
Center for advanced research in sleep medicine, Montreal
Axe Neurosciences [CHU Québec]
Sharon Hassin-Baer
  • Fonction : Auteur
Sackler Faculty of Medicine
Columbia University College of Physicians and Surgeons
Chaim Sheba Medical Center
Jacques Montplaisir
  • Fonction : Auteur
Center for advanced research in sleep medicine, Montreal
Research Center of CIUSSS of the North of Montreal and Department of Psychiatry, University of Montreal, Montreal, Quebec, Canada
Nicolas Dupré
Axe Neurosciences [CHU Québec]
Laval University Medical center
Guy Rouleau
Department of Human Genetics [Montréal]
Montreal Neurological Institute and Hospital
McGill University Health Center [Montreal]
Edward Fon
  • Fonction : Auteur
Montreal Neurological Institute and Hospital
McGill University Health Center [Montreal]
Jean-François Trempe
  • Fonction : Auteur
Department of Pharmacology and Therapeutics [Montréal]
Guillaume Lamoureux
  • Fonction : Auteur
Concordia University [Montreal]
Centre for Research in Molecular Modeling and Department of Chemistry & Biochemistry
PROTEO, The Quebec Network for Research on Protein Function, Engineering, and Applications
Center for Computational and Integrative Biology [Camden]
Roy Alcalay
  • Fonction : Auteur
Columbia University College of Physicians and Surgeons
Center for advanced research in sleep medicine, Montreal
Ziv Gan-Or
Department of Human Genetics [Montréal]
Montreal Neurological Institute and Hospital
Department of Neurology and Neurosurgery [Montreal]

Résumé

The TMEM175/GAK/DGKQ locus is the 3rd strongest risk locus in genome-wide association studies of Parkinson disease (PD). We aimed to identify the specific disease-associated variants in this locus, and their potential implications. Methods: Full sequencing of TMEM175/GAK/DGKQ followed by genotyping of specific associated variants was performed in PD (n = 1,575) and rapid eye movement sleep behavior disorder (RBD) patients (n = 533) and in controls (n = 1,583). Adjusted regression models and a meta-analysis were performed. Association between variants and glucocerebrosidase (GCase) activity was analyzed in 715 individuals with available data. Homology modeling, molecular dynamics simulations, and lysosomal localization experiments were performed on TMEM175 variants to determine their potential effects on structure and function. Results: Two coding variants, TMEM175 p.M393T (odds ratio [OR] = 1.37, p = 0.0003) and p.Q65P (OR = 0.72, p = 0.005), were associated with PD, and p.M393T was also associated with RBD (OR = 1.59, p = 0.001). TMEM175 p.M393T was associated with reduced GCase activity. Homology modeling and normal mode analysis demonstrated that TMEM175 p.M393T creates a polar side-chain in the hydrophobic core of the transmembrane, which could destabilize the domain and thus impair either its assembly, maturation, or trafficking. Molecular dynamics simulations demonstrated that the p.Q65P variant may increase stability and ion conductance of the transmembrane protein, and lysosomal localization was not affected by these variants.

Domaines

Génétique humaine Neurosciences [q-bio.NC]

Nathalie Salvy-Córdoba  : Connectez-vous pour contacter le contributeur

https://hal.umontpellier.fr/hal-03374526

Soumis le : mardi 12 octobre 2021-10:53:39

Dernière modification le : jeudi 14 mars 2024-14:32:08

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Dates et versions

hal-03374526 , version 1 (12-10-2021)

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Citer

Lynne Krohn, Tuğba Nur Öztürk, Benoît Vanderperre, Bouchra Ouled Amar Bencheikh, Jennifer Ruskey, et al.. Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. Annals of Neurology, 2019, 87 (1), pp.139-153. ⟨10.1002/ana.25629⟩. ⟨hal-03374526⟩

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