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Chapitre D'ouvrage Année : 2006

Peptide Carriers for Protein Transduction: How to Generate a Drug from Your Favorite Protein

Résumé

The development of peptide-drugs and therapeutic proteins is limited by the poor permeability and the selectivity of the cell membrane. We have developed a new peptide-based strategy (Pep-1) for protein transduction into cells. Pep-1 is an amphipathic peptide consisting of a hydrophobic domain and a hydrophilic lysine-rich domain. Pep-1 efficiently delivers a variety of fully biologically active peptides and proteins into cells, without the need for prior chemical cross-linking or denaturation steps. The mechanism through which Pep-1 delivers macromolecules does not involve the endosomal pathway and the dissociation of the Pep-1/macromolecule particle occurs immediately after it crosses the cell membrane. Pep-1 has recently been applied to the screening of therapeutic peptides in vivo and presents several advantages: stability in physiological buffer, lack of toxicity and of sensitivity to serum. Pep-1 technology could contribute significantly to the development of fundamental and therapeutic applications.
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hal-03156704 , version 1 (02-03-2021)

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M. Morris, J. Depollier, S. Deshayes, F. Heitz, G. Divita. Peptide Carriers for Protein Transduction: How to Generate a Drug from Your Favorite Protein. Polymeric Drug Delivery : Science & Application, pp.155-165, 2006, 9780841239180. ⟨10.1021/bk-2006-0923.ch011⟩. ⟨hal-03156704⟩
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