Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Proteomes Année : 2015

Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases

Résumé

Probing the dynamic activities of protein kinases in real-time in living cells constitutes a major challenge that requires specific and sensitive tools tailored to meet the particular demands associated with cellular imaging. The development of genetically-encoded and synthetic fluorescent biosensors has provided means of monitoring protein kinase activities in a non-invasive fashion in their native cellular environment with high spatial and temporal resolution. Here, we review existing technologies to probe different dynamic features of protein kinases and discuss limitations where new developments are required to implement more performant tools, in particular with respect to infrared and near-infrared fluorescent probes and strategies which enable improved signal-to-noise ratio and controlled activation of probes.
Fichier principal
Vignette du fichier
proteomes-03-00369-v2.pdf (2.43 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03032172 , version 1 (26-03-2021)

Identifiants

Citer

Juan González-Vera, May Morris. Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases. Proteomes, 2015, 3 (4), pp.369-410. ⟨10.3390/proteomes3040369⟩. ⟨hal-03032172⟩
33 Consultations
43 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More