Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Science Advances Année : 2020

Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping

Résumé

Polycomb repressive complexes 1 and 2 have been historically described as transcriptional repressors, but recent reports suggest that PRC1 might also support activation, although the underlying mechanisms remain elusive. Here, we show that stage-specific PRC1 binding at a subset of active promoters and enhancers during Drosophila development coincides with the formation of three-dimensional (3D) loops, an increase in expression during development and repression in PRC1 mutants. Dissection of the dachshund locus indicates that PRC1-anchored loops are versatile architectural platforms that persist when surrounding genes are transcriptionally active and fine-tune their expression. The analysis of RING1B binding profiles and 3D contacts during neural differentiation in mice suggests that this role is conserved in mammals.
Fichier principal
Vignette du fichier
eaax4001.full.pdf (5.39 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02896366 , version 1 (10-07-2020)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

V. Loubiere, G. L. Papadopoulos, Q. Szabo, A. M. Martinez, Giacomo Cavalli. Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping. Science Advances , 2020, 6 (2), pp.eaax4001. ⟨10.1126/sciadv.aax4001⟩. ⟨hal-02896366⟩
20 Consultations
103 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More