Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Stem Cell Research Année : 2019

Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene

Résumé

Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 58 year-old woman suffering from Alzheimer's disease and carrying a D694N mutation on Amyloid precursor protein (APP). Fibroblasts were reprogrammed into iPSC using the integration-free Sendai Virus which allows the expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. The cells have the corresponding mutation and present a normal karyotype. The reported APP-D694N iPSC line may be used to model and study human AD pathology in vitro.
Fichier principal
Vignette du fichier
1-s2.0-S1873506119300686-main.pdf (1.05 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02863304 , version 1 (10-06-2020)

Identifiants

Citer

L. Auboyer, C. Monzo, D. Wallon, A. Rovelet-Lecrux, A. Gabelle, et al.. Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene. Stem Cell Research, 2019, 37, pp.101438. ⟨10.1016/j.scr.2019.101438⟩. ⟨hal-02863304⟩
54 Consultations
90 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More