ATRX Plays a Key Role in Maintaining Silencing at Interstitial Heterochromatic Loci and Imprinted Genes - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2015

ATRX Plays a Key Role in Maintaining Silencing at Interstitial Heterochromatic Loci and Imprinted Genes

Résumé

Histone H3.3 is a replication-independent histone variant, which replaces histones that are turned over throughout the entire cell cycle. H3.3 deposition at euchromatin is dependent on HIRA, whereas ATRX/Daxx deposits H3.3 at pericentric heterochromatin and telomeres. The role of H3.3 at heterochromatic regions is unknown, but mutations in the ATRX/Daxx/H3.3 pathway are linked to aberrant telomere lengthening in certain cancers. In this study, we show that ATRX-dependent deposition of H3.3 is not limited to pericentric heterochromatin and telomeres but also occurs at heterochromatic sites throughout the genome. Notably, ATRX/H3.3 specifically localizes to silenced imprinted alleles in mouse ESCs. ATRX KO cells failed to deposit H3.3 at these sites, leading to loss of the H3K9me3 heterochromatin modification, loss of repression, and aberrant allelic expression. We propose a model whereby ATRX-dependent deposition of H3.3 into heterochromatin is normally required to maintain the memory of silencing at imprinted loci.
Fichier principal
Vignette du fichier
ATRX Plays a Key Role in Maintaining Silencing atInterstitial Heterochromatic Loci and ImprintedGenes-s2.0-S2211124715003058-main.pdf (2.9 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02539962 , version 1 (28-05-2021)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Hsiao p.J. Voon, Jim r. Hughes, Christina Rode, Inti a. De la rosa-Velázquez, Thomas Jenuwein, et al.. ATRX Plays a Key Role in Maintaining Silencing at Interstitial Heterochromatic Loci and Imprinted Genes. Cell Reports, 2015, 11 (3), pp.405-418. ⟨10.1016/j.celrep.2015.03.036⟩. ⟨hal-02539962⟩
115 Consultations
35 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More