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Freezing and storage at −20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis

Abstract : Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.
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https://hal.umontpellier.fr/hal-02506267
Contributeur : Nathalie Salvy-Cordoba <>
Soumis le : jeudi 12 mars 2020 - 11:22:08
Dernière modification le : jeudi 28 mai 2020 - 03:32:53

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Laurence Delhaes, Denis Filisetti, Marie-Pierre Brenier-Pinchart, Hervé Pelloux, Hélène Yera, et al.. Freezing and storage at −20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis. Diagnostic Microbiology and Infectious Disease, Elsevier, 2014, 80 (3), pp.197-199. ⟨10.1016/j.diagmicrobio.2014.08.007⟩. ⟨hal-02506267⟩

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