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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Abstract : Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in vivo-rapidly, repetitively, and at a high resolution. This protocol describes OCT imaging in the mouse retina as a powerful tool to study optic neuropathies (OPN). The OCT system is an interferometry-based, non-invasive alternative to common post mortem histological assays. It provides a fast and accurate assessment of retinal thickness, allowing the possibility to track changes, such as retinal thinning or thickening. We present the imaging process and analysis with the example of the Opa1delTTAG mouse line. Three types of scans are proposed, with two quantification methods: standard and homemade calipers. The latter is best for use on the peripapillary retina during radial scans; being more precise, is preferable for analyzing thinner structures. All approaches described here are designed for retinal ganglion cells (RGC) but are easily adaptable to other cell populations. In conclusion, OCT is efficient in mouse model phenotyping and has the potential to be used for the reliable evaluation of therapeutic interventions.
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https://hal.umontpellier.fr/hal-02478978
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Soumis le : vendredi 14 février 2020 - 11:42:37
Dernière modification le : vendredi 15 mai 2020 - 12:22:08
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Jolanta Jagodzinska, Emmanuelle Sarzi, Mélanie Cavalier, Marie Seveno, Volker Baecker, et al.. Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo. Journal of visualized experiments : JoVE, JoVE, 2017, ⟨10.3791/55865⟩. ⟨hal-02478978⟩

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