Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization

Abstract : Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which is of major public health concern. ZIKV infection is recognized as the cause of congenital Zika disease and other neurological defects, with no specific prophylactic or therapeutic treatments. As the humoral immune response is an essential component of protective immunity, there is an urgent need for effective vaccines that confer protection against ZIKV infection. In the present study, we evaluate the immunogenicity of chimeric viral clone ZIKBeHMR-2, in which the region encoding the structural proteins of the African strain MR766 backbone was replaced with its counterpart from the epidemic strain BeH819015. Three amino-acid substitutions I152T, T156I, and H158Y were introduced in the glycan loop of the E protein (E-GL) making ZIKBeHMR-2 a non-glycosylated virus. Adult BALB/c mice inoculated intraperitoneally with ZIKBeHMR-2 developed anti-ZIKV antibodies directed against viral proteins E and NS1 and a booster dose increased antibody titers. Immunization with ZIKBeHMR-2 resulted in a rapid production of neutralizing anti-ZIKV antibodies. Antibody-mediated ZIKV neutralization was effective against viral strain MR766, whereas epidemic ZIKV strains were poorly sensitive to neutralization by anti-ZIKBeHMR-2 immune sera. From our data, we propose that the three E-GL residues at positions E-152, E-156, and E-158 greatly influence the accessibility of neutralizing antibody epitopes on ZIKV.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [46 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02477822
Contributeur : Mélanie Karli <>
Soumis le : jeudi 13 février 2020 - 15:52:17
Dernière modification le : vendredi 13 mars 2020 - 11:54:49
Archivage à long terme le : : jeudi 14 mai 2020 - 16:22:31

Fichier

vaccines-07-00055-v2.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Etienne Frumence, Wildriss Viranaicken, Sandra Bos, Maria-Teresa Alvarez-Martinez, Marjolaine Roche, et al.. A Chimeric Zika Virus between Viral Strains MR766 and BeH819015 Highlights a Role for E-glycan Loop in Antibody-mediated Virus Neutralization. Vaccines, MDPI, 2019, 7 (2), pp.55. ⟨10.3390/vaccines7020055⟩. ⟨hal-02477822⟩

Partager

Métriques

Consultations de la notice

162

Téléchargements de fichiers

230