Phosphorylated Tyr142 β-catenin localizes to centrosomes and is regulated by Syk

Abstract : β-catenin is a central component of adherent junctions and a key effector of canonical Wnt signaling, in which dephosphorylated Ser/Thr β-catenin regulates gene transcription. β-catenin phosphorylation at Tyr142 (PTyr142 β-catenin), which is induced by receptor and Src family Tyr kinases, represents a previously described β-catenin switch from adhesive to migratory roles. In addition to classical β-catenin roles, phosphorylated Ser/Thr β-catenin and total β-catenin were involved in centrosomal functions, including mitotic spindle formation and centrosome separation. Here we find that PTyr142 β-catenin is present in centrosomes in non-transformed and glioblastoma cells and that, in contrast to the Ser/Thr phosphorylated β-catenin, PTyr142 β-catenin centrosomal levels drop in mitosis. Furthermore, we show that the inhibitor of Spleen Tyrosine Kinase (Syk) piceatannol decreases centrosomal PTyr142 β-catenin levels, indicating that Syk regulates centrosome PTyr142 β-catenin. Our findings suggest that PTyr142 β-catenin and Syk may regulate centrosomal cohesion. This study highlights the contribution of different phosphorylated β-catenin forms to the cell and centrosome cycles.
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https://hal.umontpellier.fr/hal-02281444
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Soumis le : lundi 9 septembre 2019 - 11:21:40
Dernière modification le : mardi 10 septembre 2019 - 01:19:43

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Deepshikha Bhardwaj, Mireia Náger, Anna Visa, Marta Sallán, Peter Coopman, et al.. Phosphorylated Tyr142 β-catenin localizes to centrosomes and is regulated by Syk. Journal of Cellular Biochemistry, Wiley, 2018, 119 (4), pp.3632-3640. ⟨10.1002/jcb.26571⟩. ⟨hal-02281444⟩

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