Non-steroidal anti-inflammatory drug diclofenac disturbs the prostaglandin system in the marine mussel Mytilus galloprovincialis

Abstract : Human pharmaceuticals, such as nonsteroidal anti-inflammatory drugs (NSAIDs), are an emerging environmental threat to marine organisms. Bioaccumulation in aquatic organisms along with possible ecotoxicological effects may then be of concern, as shown by the inclusion of diclofenac (DCF) in the first watch list by the EU Water Framework Directive. It is possible to hypothesize that these compounds will be pharmacologically active in organisms in which the drug targets are expressed and functional. In humans, NSAIDs act through inhibition of cyclooxygenase (COX) conversion of arachidonic acid into prostaglandins. PGs are conserved lipid signaling molecules that mediate a wide array of biological functions [1]. There have been many reports of PG biosynthesis in a broad range of invertebrates, including bivalves [2]. Therefore, modulation of PG production in marine bivalves following exposure to DCF is worth questioning. The objective of this study was thus to investigate whether diclofenac (DCF) affects PG levels in marine mussels Mytilus galloprovincialis. An experiment was carried out whereby marine mussels were exposed to DCF for 48 h to 1 and 100 µg/L DCF. A specific and sensitive analytical method using liquid chromatography tandem mass spectrometry was developed to quantify DCF accumulation in marine mussel tissues. The developed method could also clearly identify and quantify prostaglandins PGE2, PGD2 and PGF2α levels in mussel tissue and be used to assess their modulation following DCF exposure. Measured concentrations of DCF in water were relatively close to the nominal concentrations for all exposed aquaria. A low bioconcentration was calculated at 26.4 L/kg. The weak bioaccumulation of DCF observed is consistent with its possible biotransformation in the organisms, supported by the detection of hydroxy-diclofenac metabolites in exposed organisms and water. Basal PGE2 concentrations ranged from under the limit of detection (LoD) to 202 µg/kg dw. PGD2 was always found below the LoD. A downward trend in the PGE2 concentration was observed between non-exposed mussels and those exposed to 1 µg/L DCF, whereas this decrease was confirmed and statistically significant for exposure to 100 µg/L. PGF2a globally ranged from 90 to 518 µg/kg dw. No difference was observed for PGF2α levels between controls and exposed organisms.
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https://hal.umontpellier.fr/hal-02125971
Contributeur : Pascale Roussel <>
Soumis le : vendredi 10 mai 2019 - 17:27:34
Dernière modification le : jeudi 12 décembre 2019 - 10:38:02

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  • HAL Id : hal-02125971, version 1

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Frédérique Courant, Lauren Arpin-Pont, Benilde Bonnefille, S. Vacher, Marina Picot-Groz, et al.. Non-steroidal anti-inflammatory drug diclofenac disturbs the prostaglandin system in the marine mussel Mytilus galloprovincialis. 26th Annual Meeting of the Society of Environmental Toxicology and Chemistry (SETAC Europe), May 2016, Nantes, France. ⟨hal-02125971⟩

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