1H, 13C and 15N backbone chemical shift assignments of camelid single-domain antibodies against active state µ-opioid receptor - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Biomolecular NMR Assignments Année : 2017

1H, 13C and 15N backbone chemical shift assignments of camelid single-domain antibodies against active state µ-opioid receptor

Résumé

Nanobodies are single chain antibodies that have become a highly valuable and versatile tool for biomolecular and therapeutic research. One application field is the stabilization of active states of flexible proteins, among which G-protein coupled receptors (GPCRs) represent a very important class of membrane proteins. Here we present the backbone and side-chain assignment of the 1H, 13C and 15N resonances of Nb33 and Nb39, two nanobodies that recognize and stabilize the μ-opioid receptor (μOR) to opioids in its active agonist-bound conformation. In addition, we present a comparison of their secondary structures as derived from NMR chemical shifts.

Dates et versions

hal-02075373 , version 1 (21-03-2019)

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Citer

Rémy Sounier, Yinshan Yang, Joanna Hagelberger, Sébastien Granier, Héléne Démèné. 1H, 13C and 15N backbone chemical shift assignments of camelid single-domain antibodies against active state µ-opioid receptor. Biomolecular NMR Assignments, 2017, 11 (1), pp.117-121. ⟨10.1007/s12104-017-9733-z⟩. ⟨hal-02075373⟩
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