Simultaneous vitality and DNA-fragmentation measurement in spermatozoa of smokers and non-smokers - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Cytometry Part B: Clinical Cytometry Année : 2015

Simultaneous vitality and DNA-fragmentation measurement in spermatozoa of smokers and non-smokers

Résumé

BACKGROUND: Because cigarette smoke is a powerful ROS producer, we hypothesized that the spermatozoa of smokers would be more at risk of having increased DNA fragmentation than spermatozoa of non-smoking men. METHODS: A cross-sectional study was performed on consenting smokers and non-smokers, consulting in an infertility clinic for routine sperm analysis. The application of a novel TUNEL assay coupled to a vitality marker, LIVE/DEAD®, allowed both DNA fragmentation and viability measurement within spermatozoa of participants to be analyzed by flow cytometry. RESULTS: The coupled vitality-DNA fragmentation analysis revealed that non-smokers and smokers, respectively presented medians of 3.6% [0.6-36.8] and 3.3% [0.9-9.6] DNA fragmented spermatozoa among the living spermatozoa population (P > 0.05). CONCLUSION: No deleterious effect of smoking on spermatozoa was found in our study. More studies concerning potential mutagenic capacities of cigarette smoke on spermatozoa are necessary. In addition, the coupled vitality-DNA fragmentation analysis may orient Assisted Reproductive Technology teams when confronted with patients having a high percentage of DNA-fragmented living spermatozoa.
Fichier non déposé

Dates et versions

hal-01939521 , version 1 (29-11-2018)

Identifiants

Citer

A. de Bantel, J. Fleury-Feith, C. Poirot, I. Berthaut, C. Garcin, et al.. Simultaneous vitality and DNA-fragmentation measurement in spermatozoa of smokers and non-smokers. Cytometry Part B: Clinical Cytometry, 2015, 88 (2), pp.120-124. ⟨10.1002/cyto.b.21185⟩. ⟨hal-01939521⟩
37 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More