Isolation of ten microsatellite markers using a pyrosequencing procedure and cross-priming in the Salaria genus
Résumé
Ten new microsatellite markers were isolated
from the freshwater blenny, Salaria fluviatilis. These loci
were characterised for three distinct populations (Var
River and Lake Bourget in France, and Lake Kinneret in
Israel). S. fluviatilis populations in the North-West of the
Mediterranean Sea are all polymorphic and under Hardy–
Weinberg equilibrium. These microsatellite markers are
suggested for studying the genetic structure in this area.
Seven of the ten microsatellite markers are applicable for
all the freshwater blenny populations of the entire Mediterranean
basin. Cross-species characterisation was also
performed on the peacock blenny, Salaria pavo. Seven of
the ten microsatellite loci exhibited successful PCR
amplification on this latter species. Polymorphism is
observed on four of these seven loci.