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Identification of polarized macrophage subsets in zebrafish

Abstract : While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa: eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and Escherichia coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on Fluorescence Activated Cell Sorting (FACS)-sorted tnfa + and tnfa − macrophages showed that they, respectively, expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa + macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
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https://hal.umontpellier.fr/hal-01834168
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Dernière modification le : vendredi 15 mai 2020 - 12:22:10
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elife-07288-v2.pdf
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Distributed under a Creative Commons Paternité 4.0 International License

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Mai Nguyen-Chi, Béryl Laplace-Builhé, Jana Travnickova, Patricia Luz-Crawford, Gautier Tejedor, et al.. Identification of polarized macrophage subsets in zebrafish. eLife, eLife Sciences Publication, 2015, 4, ⟨10.7554/eLife.07288⟩. ⟨hal-01834168v1⟩

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