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Communication Dans Un Congrès Année : 2023

Imperatorin modulates glucose-induced insulin secretion


Pancreatic β-cell function and notably glucose-induced insulin secretion play a pivotal role in glucose homeostasis. We have previously shown that natural molecules like flavonoids or ellagitannin metabolites potentiate glucose-induced insulin secretion through a mechanism implicating the activation of L-type Ca2 + (CaV) channels and ERK1/2 kinase. In this study, we isolated a coumarin imperatorin from the roots of Angelica archangelica L. and investigated its effect on insulin secretion modulation. Material and methods: Imperatorin was isolated from the roots of A. archangelica L., through Soxhlet extraction in n-hexane, and column and preparative chromatography methods. The structure of the compound was analyzed and confirmed using High-Pressure Liquid Chromatography Diode Array Detector Electrospray Ionization Tandem Mass Spectrometry and Nuclear Magnetic Resonance methods. Pharmacological experiments were performed INS-1 β-cell line. Insulin release was quantified by the homogeneous time-resolved fluorescence (HTRF) method. Mechanism of action will be studied using western blot and/or patch clamp techniques. Results: The effects of imperatorin have been tested on insulin secretion in INS-1 cells. Increasing concentrations were tested under basal (1.4mM) or stimulating (8.3mM) glucose conditions. Under basal condition, we observed a modest increase in insulin secretion at the highest concentration. As expected, 8.3mM glucose induced a significant insulin secretion. Imperatorin potentiated 8.3mM glucose-induced insulin secretion in a concentration-dependent manner. Regarding the mechanism of action, in relation of previous results, the effects of imperatorin on ERK1/2 activation and CaV currents are under investigation in INS-1 cells. Discussion/Conclusion: Imperatorin may modulate insulin secretion in pancreatic β-cells in a glucose dependent manner.
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Dates et versions

hal-04601920 , version 1 (05-06-2024)


  • HAL Id : hal-04601920 , version 1


Andrzej Patyra, Jérémie Neasta, Justine Vaillé, Sylvie Péraldi-Roux, A Kiss, et al.. Imperatorin modulates glucose-induced insulin secretion. Annual Meeting of French Society of Pharmacology and Therapeutics, Jun 2023, Limoges (France), France. pp.215-229. ⟨hal-04601920⟩
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