GKAP-DLC2 interaction organizes the postsynaptic scaffold complex to enhance synaptic NMDA receptor activity - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2012

GKAP-DLC2 interaction organizes the postsynaptic scaffold complex to enhance synaptic NMDA receptor activity

Résumé

At glutamatergic brain synapses, scaffolding proteins regulate receptor location and function. The targeting and organization of scaffolding proteins in the postsynaptic density (PSD) is poorly understood, but it is known that a core protein of the glutamatergic receptor postsynaptic scaffold complex, guanylate-kinase-associated protein (GKAP) interacts with dynein light chain 2 (DLC2, also known as DYNLL2), a protein associated with molecular motors. In the present study, we combined BRET imaging, immunostaining and electrophysiological recording to assess the role of the GKAP-DLC2 interaction in the functional organization of the glutamatergic synapse. We found that GKAP-DLC2 interaction in dendritic spine stabilizes scaffolding protein expression at the PSD and enhances synaptic NMDA receptor activity. Moreover, the GKAP-DLC2 functional interaction is favored by sustained synaptic activity. These data identify a regulatory pathway of synaptic transmission that depends on activity-induced remodelling of the postsynaptic scaffold protein complex.
Fichier principal
Vignette du fichier
Moutin et al - 2012.pdf (1.92 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03696191 , version 1 (15-06-2022)

Identifiants

Citer

Enora Moutin, F. Raynaud, Laurent Fagni, Julie Perroy. GKAP-DLC2 interaction organizes the postsynaptic scaffold complex to enhance synaptic NMDA receptor activity. Journal of Cell Science, 2012, 125 (8), pp.2030-2040. ⟨10.1242/jcs.098160⟩. ⟨hal-03696191⟩
9 Consultations
41 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More