NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2021

NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

(1) , (1) , (1) , (1) , (2) , (2) , (1) , (3) , (4) , (1) , (1, 5) , (1)
1
2
3
4
5
Marine Pratlong
Dany Severac
  • Fonction : Auteur

Résumé

Fish species, such as zebrafish ( Danio rerio ), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3 -positive cell population within the regenerating fin. Genetic depletion of these foxd3 -positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3 -positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3 -positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.
Fichier principal
Vignette du fichier
s41467-021-26422-5.pdf (2.64 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03646943 , version 1 (20-04-2022)

Licence

Paternité - CC BY 4.0

Identifiants

Citer

Béryl Laplace-Builhé, Audrey Barthelaix, Said Assou, Candice Bohaud, Marine Pratlong, et al.. NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration. Nature Communications, 2021, 12 (1), pp.6336. ⟨10.1038/s41467-021-26422-5⟩. ⟨hal-03646943⟩
62 Consultations
25 Téléchargements

Altmetric

Partager

Gmail Facebook Twitter LinkedIn More