Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Cells Année : 2020

Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology

Résumé

Recent advances in genome engineering based on the CRISPR/Cas9 technology have revolutionized our ability to manipulate genomic DNA. Its use in human pluripotent stem cells (hPSCs) has allowed a wide range of mutant cell lines to be obtained at an unprecedented rate. The combination of these two groundbreaking technologies has tremendous potential, from disease modeling to stem cell-based therapies. However, the generation, screening and molecular characterization of these cell lines remain a cumbersome and multi-step endeavor. Here, we propose a pipeline of strategies to efficiently generate, sub-clone, and characterize CRISPR/Cas9-edited hPSC lines in the function of the introduced mutation (indels, point mutations, insertion of large constructs, deletions).
Fichier principal
Vignette du fichier
cells-09-01312.pdf (2.85 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-03594394 , version 1 (02-03-2022)

Licence

Paternité

Identifiants

Citer

Joffrey Mianné, Chloé Bourguignon, Chloé Nguyen Van, Mathieu Fieldès, Amel Nasri, et al.. Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology. Cells, 2020, 9 (5), pp.1312. ⟨10.3390/cells9051312⟩. ⟨hal-03594394⟩
23 Consultations
40 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More