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Article Dans Une Revue Organic & Biomolecular Chemistry Année : 2015

Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

Résumé

5′-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.

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Chimie
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Dates et versions

hal-03554795 , version 1 (03-02-2022)

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Maëva Reverte, Jean-Jacques Vasseur, Michael Smietana. Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection. Organic & Biomolecular Chemistry, 2015, 13 (43), pp.10604-10608. ⟨10.1039/C5OB01815C⟩. ⟨hal-03554795⟩
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