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Article Dans Une Revue eLife Année : 2021

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

1 Institute of Chemistry, The Hebrew University of Jerusalem
2 Heinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf]
3 UHasselt - Hasselt University
4 University of Sheffield [Sheffield]
5 Aarhus University [Aarhus]
6 St Jude Children's Research Hospital
7 Hochschule Mittweida - University of Applied Sciences
8 NIH - National Institutes of Health [Bethesda, MD, USA]
9 LMU - Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München
10 The Scripps Research Institute [La Jolla, San Diego]
11 UC - University of Cincinnati
12 University of Chicago
13 Columbia University [New York]
14 HHMI - Howard Hughes Medical Institute
15 Weizmann Institute of Science [Rehovot, Israël]
16 UCPH - University of Copenhagen = Københavns Universitet
17 SNU - Seoul National University [Seoul]
18 Korea University [Seoul]
19 University of Freiburg [Freiburg]
20 UCLA - University of California [Los Angeles]
21 TU Delft - Delft University of Technology
22 University of Oxford
23 Georgia Institute of Technology [Atlanta]
24 LLNL - Lawrence Livermore National Laboratory
25 Penn State - Pennsylvania State University
26 University Medical Center of the Johannes Gutenberg-University Mainz
27 IMB - Institute of Molecular Biology [Mainz, Germany]
28 CBS - Centre de Biochimie Structurale [Montpellier]
29 UUlm - University of Ulm
30 JHU - Johns Hopkins University
31 UZH - Universität Zürich [Zürich] = University of Zurich
32 UC San Francisco - University of California [San Francisco]
33 HUJ - The Hebrew University of Jerusalem
34 University of Warwick [Coventry]
35 University of Melbourne
36 Peking University [Beijing]
37 Northwestern University [Evanston]
Jelle Hendrix
  • Fonction : Auteur
Jingyi Fei
  • Fonction : Auteur
Achillefs Kapanidis
Jens Michaelis
  • Fonction : Auteur
Nicole Robb


Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB- Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage‘open science’ practices.
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Dates et versions

hal-03191929 , version 1 (05-11-2021)


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Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, et al.. FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices. eLife, 2021, 10, ⟨10.7554/elife.60416⟩. ⟨hal-03191929⟩
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