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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

Eitan Lerner 1 Anders Barth 2 Jelle Hendrix 3 Benjamin Ambrose 4 Victoria Birkedal 5 Scott Blanchard 6 Richard Börner 7 Hoi Sung Chung 8 Thorben Cordes 9 Timothy Craggs 4 Ashok Deniz 10 Jiajia Diao 11 Jingyi Fei 12 Ruben Gonzalez 13 Irina Gopich 8 Taekjip Ha 14 Christian Hanke 2 Gilad Haran 15 Nikos Hatzakis 16 Sungchul Hohng 17 Seok-Cheol Hong 18 Thorsten Hugel 19 Antonino Ingargiola 20 Chirlmin Joo 21 Achillefs Kapanidis 22 Harold Kim 23 Ted Laurence 24 Nam Ki Lee 17 Tae-Hee Lee 25 Edward Lemke 26, 27 Emmanuel Margeat 28 Jens Michaelis 29 Xavier Michalet 20 Sua Myong 30 Daniel Nettels 31 Thomas-Otavio Peulen 32 Evelyn Ploetz 9 Yair Razvag 33 Nicole Robb 34 Benjamin Schuler 31 Hamid Soleimaninejad 35 Chun Tang 36 Reza Vafabakhsh 37 Don Lamb 9 Claus Am Seidel 2 Shimon Weiss 20 
Abstract : Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB- Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage‘open science’ practices.
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https://hal.umontpellier.fr/hal-03191929
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Soumis le : vendredi 5 novembre 2021 - 14:20:25
Dernière modification le : vendredi 5 août 2022 - 12:31:36
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Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, et al.. FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices. eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/elife.60416⟩. ⟨hal-03191929⟩

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