The human (PsiL+mu-) proB complex: cell surface expression and biochemical structure of a putative transducing receptor. - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Blood Année : 1999

The human (PsiL+mu-) proB complex: cell surface expression and biochemical structure of a putative transducing receptor.

L Gauthier
  • Fonction : Auteur
V Guelpa-Fonlupt
  • Fonction : Auteur
M Fougereau
  • Fonction : Auteur
C Schiff
  • Fonction : Auteur

Résumé

The surrogate light chain (PsiL) associates with mu and Igalpha-Igbeta chains to form the preB-cell receptor that plays a critical role in early B-cell differentiation. Discrepancies exist in human concerning the existence of PsiL+mu- proB cells and the biochemical structure of such a proB-cell complex remains elusive. Among new antihuman VpreB monoclonal antibodies (MoAbs), 5 of the gamma kappa isotype bound to recombinant and native VpreB protein with high affinity. They recognized 4 discrete epitopes, upon which 2 were in the extra-loop fragment. Such MoAbs detected the PsiL at the cell surface of either preB or on both proB and preB cells. The previously reported SLC1/SLC2 MoAbs recognize a conformational epitope specific for the mu/PsiL association in accordance with their preB-cell reactivity. Using the proB/preB 4G7 MoAb, PsiL cell surface expression was detected on normal bone marrow, not only on CD34(-)CD19(+) preB but also on CD34(+)CD19(+) proB cells. Futhermore, this MoAb identified PsiL+mu- fresh proB leukemic cells of the TEL/AML1 type. Biochemical studies showed that, at the proB stage, the PsiL is associated noncovalently with two proteins of 105 and 130 kD. Triggering of this complex induces intracellular Ca2+ flux, suggesting that the PsiL may be involved in a new receptor at this early step of the B-cell differentiation.
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Dates et versions

hal-03041024 , version 1 (04-12-2020)

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Citer

Bénédicte Lemmers, L Gauthier, V Guelpa-Fonlupt, M Fougereau, C Schiff. The human (PsiL+mu-) proB complex: cell surface expression and biochemical structure of a putative transducing receptor.. Blood, 1999, 93 (12), pp.4336-46. ⟨10.1182/blood.V93.12.4336⟩. ⟨hal-03041024⟩
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