A study of plant remains from seven archaeological wells at Mas de Vignoles IX, near Nîmes, southern France, was used to shed light on a current gap in the archaeological record caused by erosion and modern agriculture. The analysis also explored the reliability of these sources for palaeoenvironmental and palaeoeconomical information. Significant results on the spatial organization of human habitation, economic activities, and on the environment and its exploitation were obtained for the Middle Neolithic to Roman periods. Furthermore, the Neolithic wells also provided the first early finds of fig seeds in France. The abundance of weed and ruderal plants up to the Iron Age is consistent with data from other studies and their dramatic decrease during the Roman period may have resulted from radical changes in land management. The study marks the first time, in southern France, that a group of wells from a single site have provided a complete record throughout the later prehistoric and Roman periods.