Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Highly Efficient Vertical Transmission for Zika Virus in Aedes aegypti after Long Extrinsic Incubation Time

Abstract : While the Zika virus (ZIKV) 2014–2017 pandemic has subsided, there remains active transmission. Apart from horizontal transmission to humans, the main vector Aedes aegypti can transmit the virus vertically from mother to offspring. Large variation in vertical transmission (VT) efficiency between studies indicates the influence of parameters, which remain to be characterized. To determine the roles of extrinsic incubation time and gonotrophic cycle, we deployed an experimental design that quantifies ZIKV in individual progeny and larvae. We observed an early infection of ovaries that exponentially progressed. We quantified VT rate, filial infection rate, and viral load per infected larvae at 10 days post oral infection (d.p.i.) on the second gonotrophic cycle and at 17 d.p.i. on the second and third gonotrophic cycle. As compared to previous reports that studied pooled samples, we detected a relatively high VT efficiency from 1.79% at 10 d.p.i. and second gonotrophic cycle to 66% at 17 d.p.i. and second gonotrophic cycle. At 17 d.p.i., viral load largely varied and averaged around 800 genomic RNA (gRNA) copies. Longer incubation time and fewer gonotrophic cycles promoted VT. These results shed light on the mechanism of VT, how environmental conditions favor VT, and whether VT can maintain ZIKV circulation.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [46 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02960996
Contributeur : Pascale Roussel <>
Soumis le : jeudi 8 octobre 2020 - 10:09:36
Dernière modification le : jeudi 18 février 2021 - 08:48:04

Fichier

téléchargement (9).pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Menchie Manuel, Dorothée Missé, Julien Pompon. Highly Efficient Vertical Transmission for Zika Virus in Aedes aegypti after Long Extrinsic Incubation Time. Pathogens, MDPI, 2020, 9 (5), pp.366. ⟨10.3390/pathogens9050366⟩. ⟨hal-02960996⟩

Partager

Métriques

Consultations de la notice

68

Téléchargements de fichiers

42