Generation of induced pluripotent stem cells (IRMBi001-A) from an Alzheimer's disease patient carrying a G217D mutation in the PSEN1 gene

Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 50 year-old patient suffering from Alzheimer's disease and carrying a G217D causal mutation on presenilin 1 (PSEN1). iPSCs were obtained following reprogramming using the integration-free Sendai Virus system which allows expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. iPS cells carry the patient G217D mutation and present a normal karyotype. The reported PS1-G217D iPSC line may be used to model and study human AD pathology in vitro.

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Neurosciences

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IRMB 101_p37-s2.0-S1873506118303167-main.pdf (2.12 Mo)

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https://hal.umontpellier.fr/hal-02927150

Soumis le : mercredi 9 décembre 2020-14:58:24

Dernière modification le : mercredi 10 avril 2024-08:50:03

Archivage à long terme le : mercredi 10 mars 2021-19:22:21

Dates et versions

hal-02927150 , version 1 (09-12-2020)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

HAL Id : hal-02927150 , version 1
DOI : 10.1016/j.scr.2018.101381

Citer

L. Auboyer, C. Monzo, D. Wallon, A. Rovelet-Lecrux, A. Gabelle, et al.. Generation of induced pluripotent stem cells (IRMBi001-A) from an Alzheimer's disease patient carrying a G217D mutation in the PSEN1 gene. Stem Cell Research, 2019, 34, pp.101381. ⟨10.1016/j.scr.2018.101381⟩. ⟨hal-02927150⟩

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INSERM COMUE-NORMANDIE BS UNIV-MONTPELLIER UNIROUEN GPMCND

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