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Article dans une revue

Rapid and specific DNA detection by magnetic field-enhanced agglutination assay

Abstract : The detection of DNA molecules by agglutination assays has suffered from a lack of specificity. The specificity can be improved by introducing a hybridization step with a specific probe. We developed a setting that captured biotinylated DNA targets between magnetic nanoparticles (MNPs) grafted with tetrathiolated probes and anti-biotin antibodies. The agglutination assay was enhanced using a series of magnetization cycles. This setting allowed to successfully detect a synthetic single stranded DNA with a sensitivity as low as 9 pM. We next adapted this setting to the detection of PCR products. We first developed an asymmetric pan-flavivirus amplification. Then, we demonstrated its ability to detect dengue virus with a limit of detection of 100 TCID 50 /mL. This magnetic field-enhanced agglutination assay is an endpoint readout, which benefits from the advantages of using nanoparticles that result in particular from a very reduced duration of the test; in our case it lasts less than 5 min. This approach provides a solution to develop new generation platforms for molecular diagnostics.
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https://hal.umontpellier.fr/hal-02904800
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Elena Pinchon, Fanny Leon, Nevzat Temurok, François Morvan, Jean-Jacques Vasseur, et al.. Rapid and specific DNA detection by magnetic field-enhanced agglutination assay. Talanta, Elsevier, 2020, 219, pp.121344. ⟨10.1016/j.talanta.2020.121344⟩. ⟨hal-02904800⟩

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