Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Low polymorphisms in pfact, pfugt and pfcarl genes in African Plasmodium falciparum isolates and absence of association with susceptibility to common anti-malarial drugs

Abstract : Resistance to all available anti-malarial drugs has emerged and spread including artemisinin derivatives and their partner drugs. Several genes involved in artemisinin and partner drugs resistance, such as pfcrt, pfmdr1, pfK13 or pfpm2, have been identified. However, these genes do not properly explain anti-malarial drug resistance, and more particularly clinical failures observed in Africa. Mutations in genes encoding for Plasmodium falciparum proteins, such as P. falciparum Acetyl-CoA transporter (PfACT), P. falciparum UDP-galactose transporter (PfUGT) and P. falciparum cyclic amine resistance locus (PfCARL) have recently been associated to resistance to imidazolopiperazines and other unrelated drugs. METHODS: Mutations on pfugt, pfact and pfcarl were characterized on 86 isolates collected in Dakar, Senegal and 173 samples collected from patients hospitalized in France after a travel in African countries from 2015 and 2016 to assess their potential association with ex vivo susceptibility to chloroquine, quinine, lumefantrine, monodesethylamodiaquine, mefloquine, dihydroartemisinin, artesunate, doxycycline, pyronaridine and piperaquine. RESULTS: No mutations were found on the genes pfugt and pfact. None of the pfcarl described mutations were identified in these samples from Africa. The K784N mutation was found in one sample and the K734M mutation was identified on 7.9% of all samples for pfcarl. The only significant differences in ex vivo susceptibility according to the K734M mutation were observed for pyronaridine for African isolates from imported malaria and for doxycycline for Senegalese parasites. CONCLUSION: No evidence was found of involvement of these genes in reduced susceptibility to standard anti-malarial drugs in African P. falciparum isolates.
Liste complète des métadonnées

Littérature citée [64 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02408550
Contributeur : Mélanie Karli <>
Soumis le : vendredi 13 décembre 2019 - 09:46:16
Dernière modification le : vendredi 10 janvier 2020 - 19:38:03
Document(s) archivé(s) le : samedi 14 mars 2020 - 13:20:27

Fichier

Sterkers_02.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Francis Tsombeng Foguim, Marie Gladys Robert, Mamadou Wague Gueye, Mathieu Gendrot, Silman Diawara, et al.. Low polymorphisms in pfact, pfugt and pfcarl genes in African Plasmodium falciparum isolates and absence of association with susceptibility to common anti-malarial drugs. Malaria Journal, BioMed Central, 2019, 18 (1), ⟨10.1186/s12936-019-2919-3⟩. ⟨hal-02408550⟩

Partager

Métriques

Consultations de la notice

52

Téléchargements de fichiers

81