The ongoing Rubiaceae treatment for the Flora of the Guianas prompted a detailed study of the rubiaceous taxa described by Aublet (“Fusée Aublet”), one of the earliest botanists to collect in South America. This study led to the recognition that a publication by Lanjouw and Uittien in 1940 wherein the authors reported the discovery of an additional set of Aublet specimens in the Jean-Jacques Rousseau herbarium (P-JJR) also included many previously unrecognized typifications of Aublet names. This study presents the typifications of the 26 generic and 53 specific Rubiaceae epithets introduced by Aublet in his Histoire. The new combinations Coussarea lutea and Schradera scandens are also proposed.