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Chapitre D'ouvrage Année : 2018

Construction of Synthetic Antibody Libraries

Résumé

Libraries of antibody fragments displayed on filamentous phages are now a widely used approach to isolate antibodies against virtually any target. We describe a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-of the antibody molecule. Using this protocol, libraries larger than 1010 can be constructed in a single week.
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Dates et versions

hal-02281581 , version 1 (09-09-2019)

Identifiants

Citer

Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau. Construction of Synthetic Antibody Libraries. Antibody Engineering, 1827, pp.93-108, 2018, Methods in Molecular Biology, 978-1-4939-8647-7. ⟨10.1007/978-1-4939-8648-4_5⟩. ⟨hal-02281581⟩
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