Three mushroom species—Flammulina velutipes, Paxillus involutus, and Tricholoma pardinum (Basidiomycota)—were investigated for pharmacological activities on mice after intraperitoneal and per os administration of fruiting body extract. In particular, analgesic and spasmolytic activities of the mushroom extracts as well as their effects on the central nervous system were reported. Both methanol-soluble and water-soluble residues separated from methanol extracts of fruiting bodies of mushrooms were used. The mushroom extracts did not show any particular analgesic effects. However, extracts demonstrated algogen activity, especially P. involutus. Significant spasmolytic papaverine-like activity was also observed for P. involutus, as revealed by decreasing intestinal passability and diarrhea. Extracts of P. involutus and T. pardinum produced effects on the central nervous system of mice.