An easy and rapid method using microscopy to determine herbicide effects in Poaceae weed species

Abstract : A new, easy, rapid and relatively inexpensive method using microscopy has been developed for the detection of herbicide effects in leaves of grass weed species displaying no visual signs of damage. The method has potential to be used as a tool to indicate future death of grass species due to herbicide exposure by observing phytochemical effects, i.e. early-warning effects. In the present study, Apera spica-venti (L.) Beauv., Bromus hordeaceus L., Alopecurus myosuroides Huds., Lolium perenne L. and Poa annua L. were exposed to lethal rates of four herbicides with different mode of action. The herbicides investigated were the thiocarbamate: prosulfocarb, the sulfonylurea: iodosulfuron, the aryloxyphenoxypropionate: fenoxaprop-P-ethyl and the organophosphate glyphosate. Autofluorescence of leaves was studied under a microscope using ultraviolet and blue light. The fluorescence of leaves treated to enhance flavonoids was also examined. To confirm the results, microspectrofluorometry was performed. Effects indicating future death of the grasses were observed in visually healthy leaves following treatment with prosulfocarb, glyphosate and iodosulfuron. No changes were detected following treatment with fenoxaprop-P-ethyl. After exposure to glyphosate or iodosulfuron, changes in the content of flavonoids and other compounds with a conjugation system and rigid structure and a decrease in the content of chlorophyll were detected in the leaves. Prosulfocarb treatment resulted in changes in the content of flavonoids and other compounds with a conjugation system and rigid structure and an increase in the content of chlorophyll in the leaves. The results obtained from microspectrofluorometry indicated that exposure to prosulfocarb caused a reduction in the flavonoids quercetin, naringenin and/or naringin.
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https://hal.umontpellier.fr/hal-02196416
Contributeur : Sylvie Rapior <>
Soumis le : dimanche 28 juillet 2019 - 17:39:21
Dernière modification le : lundi 18 novembre 2019 - 15:45:54

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Maibritt Hjorth, Laurence Mondolot, Bruno Buatois, Claude Andary, Sylvie Rapior, et al.. An easy and rapid method using microscopy to determine herbicide effects in Poaceae weed species. Pest Management Science, Wiley, 2006, 62 (6), pp.515-521. ⟨10.1002/ps.1194⟩. ⟨hal-02196416⟩

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