Preliminary PCR-TTGE analyses of bacterial communities associated with pollen from anemophilous trees: potential impacts on plants and human health

Françoise Fons 1 Stefaniya Hantova 2 Yasmine Hamdouche 3 Sylvie Rapior 1 Corinne Teyssier 2, 3
1 CEFE - Centre d’Ecologie Fonctionnelle et Evolutive
2 HSM - Hydrosciences Montpellier
3 UMR Qualisud - Démarche intégrée pour l'obtention d'aliments de qualité
Abstract : Pollen from wind-pollinated plants is the predominant cause of pollinosis which corresponds mainly to allergic rhinitis and conjunctivitis. Bacterial communities associated to leaves and roots surface were analysed for many years with both culture-dependent and independent methods. However, microbiota of pollen grains was rarely investigated and never with a molecular fingerprint technique. The aim of this study was to perform a preliminary analysis of the pollen microbiota by applying PCR-TTGE method. Pollen samples were collected from various anemophilous trees genera (Cephalotaxus, Cupressus, Pinus, Platanus and Quercus) in Montpellier (France) in 2013. Thorough pollen preparation was essential to a successful recovery of DNA from pollen. Regarding the results, pollen microbiota was tree genus-dependent. In addition, intra-tree genus variations were also observed. The Gammaproteobacteria class was the most represented in pollen samples whatever the tree genus. Among this class, some bacteria were recognized as phytopathogens (Pseudomonas, Erwinia) but also opportunistic human pathogens (Pseudomonas, some enterobacteria). PCR-TTGE is a suitable tool to analyse the microbiota associated with pollen responsible for phytopathogenicity or affecting the human respiratory tract.
Keywords : PCR-TTGE health pollinosis anemophilous trees pollen microbiota
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Soumis le : vendredi 26 juillet 2019 - 14:10:55
Dernière modification le : mardi 11 février 2020 - 10:10:14

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UNIV-MONTPELLIER | UNIV-AVIGNON | UNIV-MONTP3 | INRA | CEFE | CNRS | AGROPOLIS | CIRAD | IRD | BA | B3ESTE | EPHE | PSL | MONTPELLIER-SUPAGRO

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Françoise Fons, Stefaniya Hantova, Yasmine Hamdouche, Sylvie Rapior, Corinne Teyssier. Preliminary PCR-TTGE analyses of bacterial communities associated with pollen from anemophilous trees: potential impacts on plants and human health. Journal of Microbiology, Biotechnology and Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, 2018, 7 (5), pp.478-483. ⟨10.15414/jmbfs.2018.7.5.478-483⟩. ⟨hal-02195580⟩

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