BAG6/BAT3 modulates autophagy by affecting EP300/p300 intracellular localization - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Autophagy Année : 2014

BAG6/BAT3 modulates autophagy by affecting EP300/p300 intracellular localization

Résumé

We recently reported that BAG6/BAT3 (BCL2-associated athanogene 6) is essential for basal and starvation-induced autophagy in E18.5 bag6(-/-) mouse embryos and in mouse embryonic fibroblasts (MEFs) through the modulation of the EP300/p300-dependent acetylation of TRP53 and autophagy-related (ATG) proteins. We observed that BAG6 increases TRP53 acetylation during starvation and pro-autophagic TRP53-target gene expression. BAG6 also decreases the EP300 dependent-acetylation of ATG5, ATG7, and LC3-I, posttranslational modifications that inhibit autophagy. In addition, in the absence of BAG6 or when using a mutant of BAG6 exclusively located in the cytoplasm, autophagy is inhibited, ATG7 is hyperacetylated, TRP53 acetylation is abrogated, and EP300 accumulates in the cytoplasm indicating that BAG6 is involved in the regulation of the nuclear localization of EP300. We also reported that the interaction between BAG6 and EP300 occurs in the cytoplasm rather than the nucleus. Moreover, during starvation, EP300 is transported to the nucleus in a BAG6-dependent manner. We concluded that BAG6 regulates autophagy by controlling the localization of EP300 and its accessibility to nuclear (TRP53) and cytoplasmic (ATGs) substrates.

Dates et versions

hal-02168070 , version 1 (28-06-2019)

Identifiants

Citer

Salwa Sebti, C. Prebois, E. Perez-Gracia, C. Bauvy, F. Desmots, et al.. BAG6/BAT3 modulates autophagy by affecting EP300/p300 intracellular localization. Autophagy, 2014, 10 (7), pp.1341--2. ⟨10.4161/auto.28979⟩. ⟨hal-02168070⟩
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