Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering

Abstract : Stereolithography‐assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω‐methacrylate poly(d,l‐lactide‐block‐ethylene glycol‐block‐ d,l‐lactide) (MA‐PDLLA‐PEG‐PDLLA‐MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X‐ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.
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https://hal.umontpellier.fr/hal-02128283
Contributeur : Odile Hennaut <>
Soumis le : mardi 14 mai 2019 - 10:49:26
Dernière modification le : vendredi 16 août 2019 - 21:10:03

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Nina Yu. Kostina, Sébastien Blanquer, Ognen Pop‐georgievski, Khosrow Rahimi, Barbara Dittrich, et al.. Zwitterionic Functionalizable Scaffolds with Gyroid Pore Architecture for Tissue Engineering. Macromolecular Bioscience, Wiley-VCH Verlag, 2019, 19 (4), pp.1800403. ⟨10.1002/mabi.201800403⟩. ⟨hal-02128283⟩

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