Primitive macrophages control HSPC mobilization and definitive haematopoiesis - Université de Montpellier Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2015

Primitive macrophages control HSPC mobilization and definitive haematopoiesis

Résumé

In vertebrates, haematopoietic stem/progenitor cells (HSPCs) first emerge in the aorta-gonad-mesonephros (AGM) before colonizing transitory and subsequently definitive haematopoietic organs allowing haematopoiesis throughout adult life. Here we identify an unexpected primitive macrophage population accumulated in the dorsal mesenteric mesoderm surrounding the dorsal aorta of the human embryo and study its function in the transparent zebrafish embryo. Our study reveals dynamic interactions occurring between the HSPCs and primitive macrophages in the AGM. Specific chemical and inducible genetic depletion of macrophages or inhibition of matrix metalloproteinases (Mmps) leads to an accumulation of HSPCs in the AGM and a decrease in the colonization of haematopoietic organs. Finally, in vivo zymography demonstrates the function of primitive macrophages in extracellular matrix degradation, which allows HSPC migration through the AGM stroma, their intravasation, leading to the colonization of haematopoietic organs and the establishment of definitive haematopoiesis.
Fichier principal
Vignette du fichier
TravnickovaNatureCommun2015_7227.pdf (1.97 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02087021 , version 1 (01-04-2019)

Identifiants

Citer

Jana Travnickova, Vanessa Tran Chau, Emmanuelle Julien, Julio Mateos-Langerak, Catherine Gonzalez, et al.. Primitive macrophages control HSPC mobilization and definitive haematopoiesis. Nature Communications, 2015, 6, pp.6227. ⟨10.1038/ncomms7227⟩. ⟨hal-02087021⟩
178 Consultations
111 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More