A multicellular way of life for a multipartite virus

Abstract : A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the viral population. How multipartite viruses manage to efficiently infect individual cells with all segments, thus with the whole genome information, is a long-standing but perhaps deceptive mystery. By localizing and quantifying the genome segments of a nanovirus in host plant tissues we show that they rarely co-occur within individual cells. We further demonstrate that distinct segments accumulate independently in different cells and that the viral system is functional through complementation across cells. Our observation deviates from the classical conceptual framework in virology and opens an alternative possibility (at least for nanoviruses) where the infection can operate at a level above the individual cell level, defining a viral multicellular way of life.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [32 références]  Voir  Masquer  Télécharger

https://hal.umontpellier.fr/hal-02082621
Contributeur : Philip Agnew <>
Soumis le : jeudi 28 mars 2019 - 12:43:17
Dernière modification le : mercredi 29 mai 2019 - 10:56:23
Document(s) archivé(s) le : samedi 29 juin 2019 - 14:09:41

Fichier

elife-43599-v2.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Anne Sicard, Elodie Pirolles, Romain Gallet, Marie-Stéphanie Vernerey, Michel Yvon, et al.. A multicellular way of life for a multipartite virus. eLife, eLife Sciences Publication, 2019, 8, pp.e43599. ⟨10.7554/eLife.43599⟩. ⟨hal-02082621⟩

Partager

Métriques

Consultations de la notice

69

Téléchargements de fichiers

34