Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells

Abstract : Metabotropic glutamate receptors (mGluRs) are mandatory dimers playing important roles in regulating CNS function. Although assumed to form exclusive homodimers, 16 possible heterodimeric mGluRs have been proposed but their existence in native cells remains elusive. Here, we set up two assays to specifically identify the pharmacological properties of rat mGlu heterodimers composed of mGlu2 and 4 subunits. We used either a heterodimer-specific conformational LRET-based biosensor or a system that guarantees the cell surface targeting of the heterodimer only. We identified mGlu2-4 specific pharmacological fingerprints that were also observed in a neuronal cell line and in lateral perforant path terminals naturally expressing mGlu2 and mGlu4. These results bring strong evidence for the existence of mGlu2-4 heterodimers in native cells. In addition to reporting a general approach to characterize heterodimeric mGluRs, our study opens new avenues to understanding the pathophysiological roles of mGlu heterodimers.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.umontpellier.fr/hal-02073201
Contributeur : Sandrine Beraud <>
Soumis le : mardi 19 mars 2019 - 16:32:11
Dernière modification le : mardi 28 mai 2019 - 14:12:02

Lien texte intégral

Identifiants

Collections

Citation

David Moreno Delgado, Thor Møller, Jeanne Ster, Jesús Giraldo, Damien Maurel, et al.. Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. eLife, eLife Sciences Publication, 2017, 6, ⟨10.7554/eLife.25233⟩. ⟨hal-02073201⟩

Partager

Métriques

Consultations de la notice

20