Proteome Analysis of Urticating Setae From Thaumetopoea pityocampa (Lepidoptera: Notodontidae)

Abstract : Thaumetopoea pityocampa (Denis & Schiffermüller) (Lepidoptera: Notodontidae) is harmful to conifer trees because of defoliation and to public health because of the release of urticating setae from the caterpillars. Contact with setae by humans and domestic animals induces dermatitis, usually localized to the exposed areas. Recent studies demonstrated the presence of a complex urticating mechanism where proteins present in the setae may play a role as activators of immune responses. Yet, limited information is available at present about the proteins occurring in the setae of T. pityocampa. Using a refined method for protein extraction from the setae, and a combination of liquid chromatography tandem-mass spectrometry (LC-MS/MS), de novo assembly of transcriptomic data, and sequence similarity searches, an extensive data set of 353 proteins was obtained. These were further categorized by molecular function, biological process, and cellular location. All the 353 proteins identified were found to match through BLAST search with at least one Lepidoptera sequence available in databases. We found the previously known allergens Tha p 1 and Tha p 2 described from T. pityocampa, as well as enzymes involved in chitin biosynthesis, one of the principal components of the setae, and serine proteases that were responsible for inflammatory and allergic reactions in other urticating Lepidoptera. This new proteomic database may allow for a better understanding of the complexity of allergenic reactions due to T. pityocampa and to other Lepidoptera sharing similar defense systems.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.umontpellier.fr/hal-02046131
Contributeur : Sandrine Beraud <>
Soumis le : vendredi 22 février 2019 - 15:36:33
Dernière modification le : mardi 28 mai 2019 - 14:12:02
Document(s) archivé(s) le : jeudi 23 mai 2019 - 15:28:19

Fichier

2017 Berardi et al. proteome J...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

A Berardi, A Pivato, A Arrigoni, A Mitali, A Trentin, et al.. Proteome Analysis of Urticating Setae From Thaumetopoea pityocampa (Lepidoptera: Notodontidae). Journal of Medical Entomology, Entomological Society of America, 2017, 54 (6), pp.1560-1566. ⟨10.1093/jme/tjx144⟩. ⟨hal-02046131⟩

Partager

Métriques

Consultations de la notice

61

Téléchargements de fichiers

106