Assessing the Impact of Mechanical Damage on Full-Thickness Porcine and Human Skin Using an In Vitro Approach

Abstract : For most xenobiotics, the rates of percutaneous absorption are limited by diffusion through the horny layer of skin. However, percutaneous absorption of chemicals may seriously increase when the skin is damaged. The aim of this work was to develop an in vitro representative model of mechanically damaged skins. The epidermal barrier was examined following exposure to a razor, a rotating brush, and a microneedle system in comparison to tape-stripping which acted as a reference. Excised full-thickness skins were mounted on a diffusion chamber in order to evaluate the effect of injuries and to mimic physiological conditions. The transepidermal water loss (TEWL) was greatly increased when the barrier function was compromised. Measurements were made for all the damaged biopsies and observed histologically by microscopy. On human and porcine skins, the tape-stripping application (0 to 40 times) showed a proportional increase in TEWL which highlights the destruction of the stratum corneum. Similar results were obtained for all cosmetic instruments. This is reflected in our study by the nonsignificant difference of the mean TEWL scores between 30 strips and mechanical damage. For a specific appreciation, damaged skins were then selected to qualitatively evaluate the absorption of a chlorogenic acid solution using fluorescence microscopy.
Liste complète des métadonnées

https://hal.umontpellier.fr/hal-01959016
Contributeur : Cécile Nowak <>
Soumis le : mardi 18 décembre 2018 - 13:49:14
Dernière modification le : mardi 28 mai 2019 - 17:04:08

Lien texte intégral

Identifiants

Collections

Citation

Hinda Dabboue, Nicolas Builles, Eric Frouin, Dan Scott, Jeanne Ramos, et al.. Assessing the Impact of Mechanical Damage on Full-Thickness Porcine and Human Skin Using an In Vitro Approach. BioMed Research International , Hindawi Publishing Corporation, 2015, 2015, pp.1-10. ⟨10.1155/2015/434623⟩. ⟨hal-01959016⟩

Partager

Métriques

Consultations de la notice

46