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Article Dans Une Revue European Journal of Clinical Microbiology and Infectious Diseases Année : 2015

Faecal carriage of carbapenemase-producing Gram-negative bacilli in hospital settings in southern France

Résumé

The emergence of carbapenemase-producing Gram-negative bacilli is a worldwide problem. To date, no study has evaluated the prevalence of faecal carriage of carbapenemase-producing and carbapenem-resistant Gram-negative bacilli (CR GNB) in France. From 1 February to 30 April 2012, we conducted a prospective, multicentre study in three University Hospitals and four General Hospitals in the south of France. The carriage of carbapenemase-producing Enterobacteriaceae (CPE) and other CR GNB was screened by both cultivation on chromID® CARBA and chromID® OXA-48 media (bioMérieux) and molecular tools [multiplex polymerase chain reaction (PCR) and NucliSENS EasyQ® KPC (bioMérieux)]. The genetic relationship between isolates was assessed by rep-PCR (DiversiLab, bioMérieux) or multilocus sequence typing (MLST). The prevalences of CR GNB and carbapenemase-producing bacteria were 2.4 % (27/1,135) and 0.4 % (n = 5), respectively. Two strains corresponded to OXA-23-producing Acinetobacter baumannii and belonged to the widespread sequence type (ST) 2/international clone II, whereas one strain was an ST15 OXA-48-producing Klebsiella pneumoniae. Two OXA-48-producers were detected exclusively by PCR. This first French study revealed the very low dissemination of carbapenemase-producing bacteria in patients attending hospitals in southern France during a non-outbreak situation. However, the increasing description of epidemic cases in this area must reinforce the use of hygiene procedures to prevent diffusion of these multidrug-resistant microorganisms.
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Dates et versions

hal-01938474 , version 1 (28-11-2018)

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A. Pantel, H. Marchandin, M.-F. Prère, A. Boutet-Dubois, N. Brieu-Roche, et al.. Faecal carriage of carbapenemase-producing Gram-negative bacilli in hospital settings in southern France. European Journal of Clinical Microbiology and Infectious Diseases, 2015, 34 (5), pp.899 - 904. ⟨10.1007/s10096-014-2298-1⟩. ⟨hal-01938474⟩
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