Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry

Yiu Yiu Lee 1 Jean-Marie Galano 2 Camille Oger 2 Claire Vigor 2 Reversat Guillaume 2 Jérôme Roy 3 Jean-Yves Le Guennec 3 Thierry Durand 2 Jetty Chung-Yung Lee 4
1 The University of Hong Kong, Hong Kong, Hong Kong SAR.
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
3 PhyMedExp - Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046]
4 School of Biological Sciences
Abstract : Oxygenated lipid mediators released from non-enzymatic peroxidation of polyunsaturated fatty acids (PUFA) are known to have functional roles in humans. Notably, among these lipid mediators, isoprostanes molecules are robust biomarkers of oxidative stress but those from n-3 PUFA are also bioactive molecules. In order to identify and assess the isoprostanes, the use of mass spectrometry (MS) for analysis is preferable and has been used for over two decades. Gas chromatography (GC) is commonly coupled to the MS to separate the derivatized isoprostanes of interest in biological samples. In order to increase the accuracy of the analytical performance, GC-MS/MS was also applied. Lately, MS or MS/MS has been coupled with high-performance liquid chromatography to assess multiple isoprostane molecules in a single biological sample without derivatization process. However, there are limitations for the use of LC-MS/MS in the measurement of plasma isoprostanes, which will be discussed in this review.
Keywords : GC–MS Isoprostanes LC–MS/MS Lipid peroxidation Neuroprostanes
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https://hal.umontpellier.fr/hal-01784397
Contributeur : Dominique Mornet <>
Soumis le : jeudi 3 mai 2018 - 14:14:21
Dernière modification le : mercredi 29 mai 2019 - 11:46:05

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UNIV-MONTPELLIER | INC-CNRS | CHIMIE | BS

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Yiu Yiu Lee, Jean-Marie Galano, Camille Oger, Claire Vigor, Reversat Guillaume, et al.. Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry. Lipids, Springer Verlag, 2016, 51 (11), pp.1217 - 1229. ⟨10.1007/s11745-016-4198-x⟩. ⟨hal-01784397⟩

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